Merck, 56575-1ML, GenElute™-LPA

General description
Click here to view the kit protocol
Application
GenElute™-LPA has been used to:
extract DNA from cyanobacterial and algal cultures
precipitate genomic DNA
extract and purify DNA from snap-frozen uveal melanoma tissues
to extract RNA from isolated CD4⁺ cells

Biochem/physiol Actions
GenElute LPA Linear Polyacrylamide is an efficient neutral carrier for precipitating picogram amounts of nucleic acids with ethanol. The nucleic acid precipitate can be collected simply by centrifugation. LPA offers several advantages for recovering DNA or studying DNA-protein interactions, relative to other carriers, such as tRNA or glycogen. tRNA interferes with DNA during phosphorylation with polynucleotide kinase and glycogen competes with protein in DNA-protein interaction studies. In contrast, LPA is completely inert. LPA is synthesized chemically and, therefore, is not contaminated with biological material. The precipitate is visible immediately upon addition of LPA, thus eliminating wait time and low temperature incubation.
The presence of LPA during ethanol precipitation results in complete recovery of fragments larger than 20 base pairs, whereas most of the DNA is lost if no carrier is used. Very short DNA fragments, less than 20 base pairs, do not coprecipitate with LPA, allowing separation of labeled DNA from unreacted nucleotides by precipitation after the labeling reaction.
Packaging
GenElute LPA is tested for nucleases, and is supplied in nuclease-free water as a 25 mg/ml solution.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC