Description
General description
B834 is the parental strain for BL21 (1). These protease-deficient hosts are methionine auxotrophs and allow high specific-activity labeling of target proteins with ³⁵S-methionine and selenomethionine for crystallography (2).
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
Genotype: F⁻ompT hsdSB(rB⁻ mB⁻) gal dcm met (DE3) pLysS (CamR)
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B834 host strains are methionine auxotrophs that allows high specific activity labeling of target proteins with 35S-methionine. T7 lysozyme expression suppresses basal T7 expression.
Features and Benefits
Allows for ³⁵S-methionine or selenomethionine labeling for crystallography studies
Deficient in lon and ompT proteases
Guaranteed transformation efficiencies of >2x10⁶ cfu/μg
Provided in a 0.2 mL volume; each vial can be used for 10 x 20μL transformations
Prepared using an optimized chemical method
Other Notes
1. Wood, W. B. (1966) J. Mol. Biol. 16, 118–133.
2. Leahy, D. J., Hendrickson, W. A., Aukhil, I., and Erickson, H. P. (1992) Science 258, 987–991.
0.4 ml1 mlComponent
•2 × 0.2 ml5 × 0.2 mlB834(DE3)pLysS Competent Cells
•2 × 2 ml4 × 2 mlSOC Medium
•2 ng2 ngTest Plasmid
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
