Description
General description
pET Expression Systems and pET
pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.
Components: pET Expression Systems
Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
10 µg pET vector DNA (for each indicated plasmid)
0.2 ml BL21 Glycerol Stock
0.2 ml BL21(DE3) Glycerol Stock
0.2 ml BL21(DE3)pLysS Glycerol Stock
0.2 ml Induction Control Glycerol Stock
Components: pET Expression Systems plus Competent Cells
pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
0.2 ml : NovaBlue Competent Cells
0.2 ml : BL21(DE3) Competent Cells
0.2 ml : BL21(DE3)pLysS Competent Cells
2 × 0.2 ml : SOC Medium
10 µl : Test Plasmid
These components are sufficient for up to 10 transformations in each host.
Purification and Detection Reagents
Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters. pET Expression System 23 The pET Expression System 23 contains 10 µg each of the four versions of pET-23 (pET-23a–d(+)). pET-23(+) is a transcription vector designed for expression from bacterial translation signals carried within a cloned insert. It therefore lacks the ribosome binding site and ATG start codon present on the pET translation vectors. A C-terminal HIS TAG® sequence is available. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB051VM.
Other Notes
10 µg : eachpET-23a-d(+) vector DNA
0.2 ml : Host bacterial strains BL21, BL21(DE3), and BL21(DE3)pLysS, glycerol stocks
0.2 ml : Induction control clone, glycerol stock
Legal Information
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Clickhere for Enduser Declaration (EUD) Form.
Toxicity: Standard Handling
