Merck, 70922-3, BugBuster® HT Protein Extraction Reagent

General description
BugBuster HT Protein Extraction Reagent combines BugBuster Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.
Application
BugBuster® HT Protein Extraction Reagent has been used:
to lyse the Escherichia coli cell pellets following centrifugation for protein expression and immunoblotting
for protein extraction from bacterial cell pellet
used to lyse the E. coli cell pellets for the expression and purification of dMIC60 (mitofilin) protein

Features and Benefits
Fast protein extraction and nucleic acid degradation
Ideal for processing various samples of different volumes
Increased extraction is ensured by the addition of rLysozyme solution

Legal Information
BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)