Merck, 71187-4, Insect PopCulture® Reagent

General description
Insect POPCULTURE® Reagent is a detergent-based lysis reagent specifically formulated for total insect cell culture extraction without the need for centrifugation. The improved method recovers both protein released into the medium and intracellular protein, increasing processing efficiency and target protein yields (Loomis 2002). It is amenable for automated expression-level screening and is fully compatible with the Ni-NTA His•Bind affinity purification method. Insect PopCulture reagent can be used for protein extraction from insect cells grown in suspension and adherent cells grown on tissue culture plates.
Features and Benefits
No need to separate cells from culture media
No need to clarify cell extracts prior to purification
Higher protein yield due to target protein recovery from both medium and cells
Direct affinity adsorption of target proteins to IMAC affinity resin from the total culture extract
Compatible with protease inhibitor cocktails
Ideal for high-throughput, expression-level screening and protein purification
Compatible with transfected and baculovirus-infected insect cell cultures

Other Notes
Loomis, K., et al. 2002. inNovations15, 16.
Legal Information
This product is covered by EP Patent 1,432,822 owned by EMD Chemicals Inc. or its Affiliates.
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
POPCULTURE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)