Description
General description
The BugBuster® Master Mix enables efficient protein purification and other related applications. It replaces the need for mechanical methods like the French press or sonication. The two available package sizes contain sufficient reagents for extracting proteins from either 20 grams or 100 grams of cell paste.
Application
BugBuster® Master Mix has been used:
to resuspend and lyse the cell pellets during protein purification
to extract proteins for immunoblotting
as a bacterial lysis buffer for the preparation of inclusion bodies
Biochem/physiol Actions
BugBuster® Protein Extraction Reagent is specifically designed to gently disrupt the cell wall of E. coli, allowing the release of soluble proteins. This unique formulation utilizes a detergent mix that can effectively perforate the cell wall without denaturing the soluble proteins. By combining BugBuster Reagent, rLysozyme solution, and Benzonase Nuclease, our BugBuster Master Mix enhances protein extraction efficiency and simplifies downstream processing of protein extracts.
Features and Benefits
Efficient lysis of Gram-positive and Gram-negative bacteria
Maximum recovery of active, soluble protein
Capable of cell wall perforation without denaturing soluble protein
Useful for the preparation of high purity inclusion bodies
Compatible with protease and phosphatase inhibitor cocktail
Offers a convenient, fast, and cost-effective solution for releasing the expressed target protein.
The Master Mix eliminates the need to dilute or perform separate addition steps.
Legal Information
BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
