Merck, 840046P-5MG, Brain PI(4,5)P2

General description
Although PI(4,5)P2 is a minor component of cell membranes, it plays a critical role as a substrate for a number of important signaling proteins. PI(4,5)P2 is an intermediate in the IP3/DAG pathway where it is hydrolyzed by phospholipase C to liberate the second messengers, inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). PI(4,5)P2 is also a substrate for PI 3-kinase where it is phosphorylated to PI(3,4,5)P3, an activator of downstream signaling components such as the protein kinase AKT.
Phosphatidylinositol 4,5-bisphosphate (PIP2) is synthesized from the precursor phosphatidylinositol 4-phosphate(PI4P). PIP2 is a phosphoinositide with three negative charges.
Application
Brain PI(4,5)P2 has been used:
in the preparation of Folch liposome
in the preparation of giant unilamellar vesicles and multilamellar lipid sheets
as a substrate for rubidium flux assay
in the biochemical reaction assay mixture for various lipid kinases like Phosphatidylinositol-3-kinase gamma (PI3Kγ), Phosphatidylinositol-3-kinase beta (PI3Kβ), Phosphatidylinositol-3-kinase gamma (PI3Kα)

Biochem/physiol Actions
Phosphatidylinositol 4,5-bisphosphate (PIP2) is a signaling phospholipid, present in the plasma membrane. It acts as a precursor for inositide 3 phosphate, diacylglycerol. PIP2 is a gating ligand for most of the ion channels. It functions as an activatory ligand for eukaryotic inward rectifier potassium (Kir) channels.
Packaging
5 mL Clear Glass Sealed Ampule (840046P-10mg)
5 mL Clear Glass Sealed Ampule (840046P-1mg)
5 mL Clear Glass Sealed Ampule (840046P-25mg)
5 mL Clear Glass Sealed Ampule (840046P-5mg)
Legal Information
Avanti Research is a trademark of Avanti Polar Lipids, LLC