Merck, 88022410-1VL, AFRC IAH-CC15

Immunogen
Bovine lymphocytes
Application
Flow cytometry, immunohistology, immunoprecipitation, complement lysis, in vivo depletion
Biochem/physiol Actions
Mouse NS1 x Mouse BALB/c spleen
The antibody is directed against WC1 antigen found on bovine CD4- CD8- and CD2- T cells which express gamma/delta TcR. The antigen has a molecular weight of 215-300kDa and is equivalent to the T19 antigen in sheep. The antibody is directed against equivalent antigen in sheep and goats.
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
RPMI 1640 + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by [email protected] to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to [email protected].