| NACRES | NA.21 |
|---|---|
| UNSPSC Code | 41105319 |
| form | solution |
| manufacturer/tradename | Calbiochem® |
| Quality Level | 100 |
| impurities | DNase, none detected |
| pH | 7.90—8.10 |
| shipped in | ambient |
Answer:It is a 1× solution containing 10 mM Tris-HCl and 1 mM EDTA at pH 8.0.
Answer:EDTA binds divalent metal ions and helps limit metal-dependent nuclease activity during nucleic acid storage.
Answer:Not necessarily. EDTA may affect magnesium-dependent enzymes, so its carryover and the reaction's buffer requirements must be considered.
Merck TE Buffer Solution pH 8.0 is a ready-to-use 1× Tris–EDTA solution for storing and handling DNA and other nucleic acids in molecular biology workflows. The formulation contains 10 mM Tris-HCl and 1 mM EDTA at pH 8.0. Tris maintains a buffered environment, while EDTA chelates divalent metal ions that can support nuclease activity. The molecular-biology-grade solution supports nucleic acid resuspension, short- and longer-term storage under appropriate laboratory conditions, and preparation of working stocks. The 100 mL format reduces routine buffer preparation while helping laboratories maintain consistent formulation between samples. TE buffer is not a PCR master mix; its EDTA content should be considered when preparing samples for magnesium-dependent enzymatic reactions.
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