Description
Application
NanoFabTx™ Biotin Lipid Mix is a ready-to-use nanoformulation lipid mixture for the synthesis of biotin-functionalized liposomes. This lipid mix enables users to encapsulate a wide variety of small molecules for targeting and immunotherapy applications without the need for lengthy trial-and-error optimization.
This product includes a curated lyophilized lipid mixture containing rationally selected lipids in precise ratios that have been optimized to achieve a desired size range of liposomes. Step-by-step protocols for the synthesis of liposomes surface-functionalized with biotin groups using extrusion and microfluidics are also included. The synthesized biotin-functionalized liposomes enable 1) selectively targeting cells which overexpress avidin-receptors (i.e. HeLa cells) and 2) the conjugation of avidin-tagged antibodies or proteins (i.e. Streptavidin) to the liposomes surface for immunotherapy applications and multi-targeting or multi-type cargo delivery.
Features and Benefits
A ready-to-use nanoformulation lipid blend for the synthesis of biotin-functionalized liposomes
Step-by-step extrusion and microfluidics protocols for liposome synthesis developed and tested by our formulation scientists
Flexible synthesis methods to create uniform and reproducible liposomes
Protocols and lipid blend optimized to make liposomes around 100 nm diameter with low polydispersity
Lipid mix optimized for avidin-tagged protein or antibody conjugation and small molecule encapsulation
Comprehensive protocols for liposome synthesis are included:
A lipid film hydration and extrusion protocol
A microfluidics protocol using commercial platforms or syringe pumps
The microfluidics protocol included with this product uses the NanoFabTx™ device kit (911593). These kits come with the microfluidics chips, fittings, and tubing required to get started with microfluidics-based synthesis (compatible microfluidics system or syringe pump required).
For more information, please refer to the protocol under the Protocol section of this page.
Legal Information
NANOFABTX is a trademark of Sigma-Aldrich Co. LLC
