Description
General description
TRI reagent® also known as TRIzol, is a monophasic solution of phenol and guanidinium isothiocyanate. It has a potential, to simultaneously solubilize biological material and denatures protein. It is widely used for deproteinizing RNA. This improved version of the single-step total RNA isolation reagent developed by Chomczynski allows a quick, economical, and efficient isolation of total RNA from different biological samples. This product is pure (A₂₆₀/A₂₈₀ ~1.8) and can be directly used in Northern blotting. Simultaneous isolation of DNA, RNA and proteins is also possible. 1 mL is sufficient for the extraction of 100 mg tissue or 10⁷ cells.
Application
TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski. The RNA isolation method based on this reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.
TRI Reagent® has been used for isolating RNA from various types of cells or tissues. It may also be used for processing tissues, cells cultured in monolayer, or cell pellets.
Features and Benefits
Easily scalable RNA isolation
Works with many sources: human, plant, yeast, bacterial, or viral
Better yields than traditional guanidine thiocyanate/cesium chloride methods
Legal Information
TRI Reagent is a registered trademark of Molecular Research Center, Inc.
