Description
General description
Poly-L-Lysine is a synthetic amino acid chain that is positively charged having one hydrobromide per unit of Lysine. Poly-L-Lysine is widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. Certain cell types secrete proteases, which can digest Poly-L-Lysine.
The molecular weight of Poly-L-Lysine for cell culture can vary significantly, with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (greater than 300,000 Da) having more binding sites per molecule. This product uses a Poly-L-Lysine of 84000 Da, yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment.
Application
Poly-L-Lysine Solution has been used to coat culture dishes to culture U2-OS cells and primary cortical neurons.
Biochem/physiol Actions
Poly-L-lysine serves as a coating substance to ensure better attachment of cells to culture surfaces. Research shows that plates coated with poly-L-lysine (PLL) demonstrated an accelerated cell growth rate. Culturing mesenchymal stem cells (MSCs) on PLL-coated substrates has been found to safeguard the stemness properties of these cells and inhibit senescence. PLL-coated plates have demonstrated enhanced MSC growth and up-regulated the expression of genes associated with cell adhesion, differentiation, proliferation, and signaling. Moreover, porous PLL-coated surfaces have shown improvements in cell proliferation and the osteogenic differentiation of MSCs.
Preparation Note
Optimal coating concentrations must be determined by the end user. Typical coating concentrations range from 10ug/ml to 100ug/ml based on cell type and application.
1. Thaw Poly-L-Lysine solution at room temperature.
2. Dilute Poly-L-Lysine solution to the desired concentration in sterile water.
3. Fully coat the cell culture surface with diluted Poly-L-Lysine solution. Use 5 mL volume for 6-cm plates and 10 mL volume for 10-cm plates and T75 flasks.
4. Allow the cell culture vessel to sit at room temperature overnight.
5. Aspirate the Poly-L-Lysine solution the following day and rinse the vessel with sterile water followed by coating with desired ECM protein.
Analysis Note
Solution is clear with no particulates present. Solution is sterile and suitable for cell culture applications.
Legal Information
NOVASEPTUM is a registered trademark of Merck KGaA, Darmstadt, Germany
