Merck, A0410-1MG, Aminoallyl-dUTP sodium salt

Application
Aminoallyl-dUTP may be used for indirect stable labeling of first-strand cDNA synthesis via polymerase chain reaction (PCR). Aminoallyl-dUTP functions as a reactive site for subsequent labeling by reactive fluorescent probes. Aminoallyl-dUTP may also be useful in the construction of reactive DNA scaffolds.
Aminoallyl-dUTP sodium salt has been used:
to add to the deoxynucleoside triphosphate mix for complementary DNA (cDNA) synthesis
for the generation of chromatin immunoprecipitation (ChIP) probes
in the reverse transcription of RNA for the synthesis of fluorescent labelled probes

Biochem/physiol Actions
Aminoallyl-dUTP sodium salt/5-(3-aminoallyl)-2′-deoxyuridine 5′-triphosphate (aa-dUTP) have been used mainly in two-step labeling approach for the synthesis of nucleic acid hybridisation probe.