Merck, A2647-5MG, Adenosine 5′-(β,γ-imido)triphosphate lithium salt hydrate

Application
Adenosine 5′-(β,γ-imido)triphosphate lithium salt hydrate has been used in a study to research the effects of monovalent cations Li⁺, Na⁺, K⁺, NH₄⁺, Rb⁺ and Cs⁺ on the solid and solution structures of the nucleic acid components. Adenosine 5′-(β,γ-imido)triphosphate lithium salt hydrate has also been used in a study to determine that antidepressants inhibit interferon-γ-induced microglial production of IL-6 and nitric oxide.
Biochem/physiol Actions
A non-hydrolyzable ATP analog. AMP-PNP competitively inhibits ATP-dependent enzyme systems, such as glutamine synthetase. It inhibits a number of enzymes which require the hydrolysis of ATP such as DNA topoisomerase II, SV40 large T-antigen helicase and a number of kinases. Blocks ATP-sensitive calcium-dependent potassium channels.
Preparation Note
A 10 mg/mL stock solution may be made, aliquoted and stored at -70 °C for up to 3 months.
Disclaimer
AMP-PNP is very unstable in acidic conditions; rapidly hydrolyzes to the phosphoramidate and inorganic phosphate.