Merck, A2917-1L, Acrylamide/Bis-acrylamide

General description
Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.
Application
Ready to reconstitute dry powder blends are accurately pre-blended to produce a 40% (w/v) stock solution for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.
Acrylamide/Bis-acrylamide has been used:
in the preparation of urea polyacrylamide gel during a modified cross-linking affinity purification protocol (cCLAP)
in microsatellite genotyping and nonfluorescent elastin (ELN) (GT)n polymorphism genotyping
in polyacrylamide gel for separation of proteins in SDS-PAGE for western blotting

Features and Benefits
Eliminates the need to weigh toxic acrylamide and bis-acrylamide.
Preparation Note
Reconstitute by slowly adding the indicated amount of distilled water to the container and dissolve contents.
To make up 100 ml of A2917, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution.
For the 1 Liter size, use 630 mL of water.