Merck, A4718-25G, Agarose

Name: Agarose
Brand: Merck
SKU: A4718-25G

Size:25 g, 3, 6-Anhydro-α-L-galacto-β-D-galactan, Molecular Biology

Available Catalog Numbers

A4718-100G A4718-25G

Catalog Number: A4718-25G

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Category

Agarose

Pack Size25 g

TagsAgarose Chemistry & Biochemicals Biochemicals

CollectionsMerck Biochemicals Collection: Trusted Lab Reagents & Buffers Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Agarose is a polymer extracted from agar or agar-bearing marine algae. This purified linear galactan hydrocolloid comprises alternating co-polymers D-galactose and 3,6-anhydro-L-galactose units connected by α-(1→3) and β-(1→4) glycosidic bonds. Agarose is highly biocompatible and possesses variable mechanical and diffusion properties. Agarose can be used as a gelling agent, to separate nucleic acids electrophoretically.
Application
Agarose has been used in the preparation of microgel slides employed in lysis and electrophoresis. It has also been used in the gel electrophoresis to separate restriction enzyme digested products in catechol-O-methyltransferase (COMT) genotyping
Ideal for the separation of small DNA fragments (e.g., PCR products) differing in size by as little as 2% and compares to the resolution of DNA in polyacrylamide gels. High resolution agarose is also ideal for resolving amplification fragment length polymorphisms (AmpFLP), short tandem repeats (STR) and tri- and tetranucleotide repeats.
Low ethidium bromide and SYBR Green background staining
Analysis Note
The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Specifications

General

UNSPSC Code	41105317
PubChem Substance ID	24890904
EC Number	232-731-8
NACRES	NA.25
MDL number	MFCD00081294
SMILES string	O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O
InChI	1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key	MJQHZNBUODTQTK-WKGBVCLCSA-N
biological source	algae (marine)
grade	Molecular Biology
form	powder
technique(s)	electrophoresis: suitable
EEO	≤0.05
mp	≤75 °C (3% gel)
transition temp	gel point ≤35 °C (3% gel)
foreign activity	DNase, RNase, NICKase, none detected
storage temp.	room temp
Quality Level	200

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Merck, A4718-25G, Agarose

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