Merck, A8582-100G, Antifoam SE-15

Name: Antifoam SE-15
Brand: Merck
SKU: A8582-100G

Size:100 g, silicone-based antifoam, aqueous emulsion for bacterial and mammalian systems

Available Catalog Numbers

A8582-100G A8582-500G

Catalog Number: A8582-100G

Pricing

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Category

Ionic Detergents

Pack Size100 g

TagsChemistry & Biochemicals Biochemicals Detergents

CollectionsMerck Biochemicals Collection: Trusted Lab Reagents & Buffers Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Antifoam SE-15 is a silicone-based product used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.
Application
Antifoam SE-15 has been used:

in the preparation of ramified rolling circle amplification (RAM) amplification buffer.
to find its effects on cell viability, virus infectivity and PCR (polymerase chain reaction) performance.
as a constituent of phosphate-buffered saline (PBS) for the determination of total antioxidant status (TAOS) by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay.
as a component of autoinduction expression culture.
as a component of cyclic adenosine monophosphate (cAMP) detection buffer.

Features and Benefits

Controls foaming in microbial culture flasks or fermentations
Non-toxic to the culture medium
Can be pumped to fermenter on an as-needed basis

Preparation Note
Antifoam SE-15 can be pre-diluted with 3-10 parts of cool water to aid in dispersion. Pre-diluted
suspensions should be used immediately.

Other Notes
Antifoam SE-15 is an aqueous emulsion containing 10% active silicon and non-ionic emulsifiers .

Specifications

General

NACRES	NA.85
UNSPSC Code	12161901
form	emulsion (aqueous)
concentration	10% (active silicon)
technique(s)	cell culture \| hybridoma: suitable
solubility	H2O: soluble (effective hot or cold.)
density	1.0 g/mL at 25 °C
application(s)	microbiology
storage temp.	room temp
suitability	(Mammalian (suspension))
Quality Level	200

Ordering

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