Merck, A9207-5ML, Avidin−Agarose from egg white

General description
Avidin comprises of 0.05% of total egg white protein. It is a glycosylated protein. It corresponds to a molecular weight of 16.5 kDa and exists as tetramer, which can bind a minimum of three biotin molecules.
Application
Avidin-Agarose from egg white has been used:
in the precipitation of biotin tagged protein by pull down assay in neuroblastoma cell line (SH-SY5Y )
in the purification of Lewis(x) sugar binding proteins from Schistosoma mansoni
in avidin bead assay for purification of labelled biotin
for capture of glycoproteins from murine cornea samples

Avidin is used for the purification of biotin containing proteins, which can enhance cellular binding and internalization of the antibody (Ab)-functionalized capsules compared with control human immunoglobulin (IgG)-functionalized capsules, suggesting these capsules can specifically interact with cancer cells through antibody/antigen recognition.
Biochem/physiol Actions
Avidin finds biotechnological applications due to its high-affinity ligand binding property. Avidin interaction with biotin through its carbohydrate chains is widely exploited in biotechnological applications. In cancer cells, avidin-biotin systems helps in the in vivo localization and drug targeting in tumor cells.
Physical form
Suspension in 50% glycerol, 0.01 M sodium phosphate, pH 7.0, containing 0.02% sodium azide.
Other Notes
One unit will bind 1.0 μg of d-biotin.