Merck, A9539-250G, Agarose

Name: Agarose
Brand: Merck
SKU: A9539-250G

Size:250 g, 3, 6-Anhydro-α-L-galacto-β-D-galactan, Agarose LE, BioReagent, Molecular Biology, low EEO

Available Catalog Numbers

A9539-100G A9539-10G A9539-250G A9539-25G A9539-500G A9539-50G

Catalog Number: A9539-250G

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Category

Agarose

Pack Size250 g

TagsAgarose Chemistry & Biochemicals Biochemicals

CollectionsMerck Biochemicals Collection: Trusted Lab Reagents & Buffers Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Agarose is a natural polysaccharide isolated from the seaweed genera Gelidium and Gracilaria. Structurally, it is a linear polymer consisting of alternating D-galactose and 3,6-anhydro-L-galactose units.
Application
Agarose has been used:

in gel electrophoresis to analyze the integrity of DNA
to prepare hydrocolloid gels and study the structural influence of gels on the release of carbohydrates
in gel electrophoresis to analyze the integrity of RNA
it is suitable for protein applications such as Ouchterlony and radial immunodiffusion (RID)

Biochem/physiol Actions
Agarose serves as a gelling agent and is extensively used in molecular biology research to separate and analyze nucleic acids by gel electrophoresis or blotting (Northern or Southern). During gelation, agarose polymers combine non-covalently resulting in a network of bundles whose pore sizes decide a gel′s molecular sieving properties. The type and the concentration of agarose influence the rate of migration of a DNA molecule through a gel. However, agarose also has attractive features that bring about a strong interest in its usage in biological applications. It is known to mimic the extracellular matrix which makes it an excellent biomaterial for tissue engineering applications. Due to its great water uptake capability agarose is suitable for cell-encapsulation. Agarose allows regulated permeation for oxygen and nutrients and is useful in cell growth, differentiation, and proliferation. Agarose is used in controlled/localized drug delivery and regenerative medicine, such as neurogenesis, angiogenesis, spermatogenesis, cartilage formation, bone regeneration, wound healing, and artificial pancreas.
Features and Benefits

Biocompatible
Agarose gels have larger pore sizes than polyacrylamide gels at low concentrations
Unlike polyacrylamide, the consistency of the gels is more solid (but also less elastic)
Possesses low ethidium bromide and SYBR Green background staining

Analysis Note
The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Other Notes
2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
For additional information on our range of Biochemicals, please complete this form.

Specifications

General

UNSPSC Code	41105317
PubChem Substance ID	24891469
EC Number	232-731-8
NACRES	NA.25
MDL number	MFCD00081294
SMILES string	O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O
InChI	1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key	MJQHZNBUODTQTK-WKGBVCLCSA-N
biological source	algae (marine)
grade	Molecular Biology
product line	BioReagent
form	powder
technique(s)	electrophoresis: suitable
impurities	≤10% moisture content
EEO	0.09-0.13
transition temp	gel point 36 °C ±1.5 °C (1.5% gel)
gel strength	≥1200 g/cm2 (1% gel)
anion traces	sulfate (SO42-): ≤0.15%
suitability	suitable for electrophoresis

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Merck, A9539-250G, Agarose

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