Merck, A9891-1G, 7-Amino-4-methylcoumarin

Application
Used as matrix for analysis of monosulfated disaccharides and as a co-matrix with 6-aza-2-thiothymidine for sulfated neutral and sialylated tri-and tetrasaccharides.
7-Amino-4-methylcoumarin has been used:
to determine cathepsin B like- activity
as a substrate for leucine aminopeptidase (LAP)
in chymotryptic assay
as a standard to detect the activation of caspase-3 during
sanguinarine-induced damage
protection by human DEAD-box DDX3

Biochem/physiol Actions
7-Amino-4-methylcoumarin is a coumarin derivative, which serves as a fluorescence reference standard to screen protease inhibitors.
Preparation Note
Soluble in DMSO (10 mg/mL), DMF, and acetone (10 mg/mL).
Other Notes
Reagent for preparing new fluorogenic AMC-based substrates for cystine aminopeptidase (and other hydrolases); used as reference compound in the enzyme assay.