Merck, APOAF-20TST, Annexin V-FITC Apoptosis Detection Kit

General description
Annexin V-FITC kit allows fluorescent detection of annexin V bound to apoptotic cells and quantitative determination by flow cytometry. The AnnexinV-FITC kit uses annexin V conjugated with fluorescein isothiocyante (FITC) to label phosphatidylserine sites on the membrane surface. The kit includes propidium iodide (PI) to label the cellular DNA in necrotic cells where the cell membrane has been totally compromised. This combination allows the differentiation among early apoptotic cells (annexin V positive, PI negative), necrotic cells (annexin V positive, PI positive), and viable cells (annexin V negative, PI negative).
Application
Annexin V-FITC Apoptosis Detection Kit was used:
in staining of LNCaP prostate cancer cells for measuring the G. lucidum extracts activity during the treatment of prostate cancer.
for tumor cell labelling to study the inhibitory activity of DBP-maf (Vitamin D binding protein-macrophage activating factor) on prostate tumor cells.
for indirect measurement of flippase activity.

Features and Benefits
Detects apoptosis earlier in the process than DNA-based assays such as TUNEL.
Rapid labeling of cells. Cell staining takes only 10 minutes.
No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
Propidium iodide secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.

Other Notes
Allow all components to reach room temperature before use.