Merck, B5525-5G, Bromophenol Blue sodium salt

General description
Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.
Application
A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.
Bromophenol blue sodium salt has been used in the preparation of protein samples for western blotting analysis. It has also been used in Drosphila fly food to aid visualization of gut for dissection.
Preparation Note
A 6x Gel Loading Buffer can be made using standard recipes, including 0.015% bromophenol blue.