Merck, B8438-250ML, Alcian Blue solution

Name: Alcian Blue solution
Brand: Merck
SKU: B8438-250ML

Size:250 mL, Alcian Blue 8XG solution, 1% in 3% acetic acid, pH 2.5

Available Catalog Numbers

B8438-250ML B8438-500ML

Catalog Number: B8438-250ML

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Category

Histological Stains

Pack Size250 mL

TagsClinical & Diagnostics Tissue Diagnostics Hematology Stains

CollectionsMerck Tissue Diagnostics – Universal Reagents for Histology & IHC Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

Application
Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated. Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2.5. Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used. Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride.
Biochem/physiol Actions
Alcian Blue is a cationic dye. It forms insoluble complexes with acidic glycosaminoglycans, thereby aiding in the quantitative determination of glycosaminoglycans. It is also used for the staining of bone and cartilage.

Specifications

General

NACRES	NA.47
UNSPSC Code	12171500
form	liquid
concentration	1% in 3% acetic acid, pH 2.5
technique(s)	microbe id \| staining: suitable
color	dark blue to very dark blue
pH range	2.4-2.6
A<sub>max</sub>	0.45-0.60 (1:400 in H2O)
application(s)	diagnostic assay manufacturinghematologyhistology
storage temp.	room temp
Quality Level	100

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