Merck, B8503-1G, 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

General description
5-bromo-4-chloro-3-indolyl phosphate (BCIP) is an histochemical phosphatase stain.
Histochemical substrate for alkaline phosphatase.
Application
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
For the colorimetric detection of alkaline phosphatase-labeled molecules.
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt has been used in in situ hybridization.
Biochem/physiol Actions
5-bromo-4-chloro-3-indolyl phosphate (BCIP) can be used to visualize the phosphatase activity. It can be used as a substrate to monitor secreted embryonic alkaline phosphatase activity in solution.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany