Merck, C1389-5G, Carbenicillin disodium salt

General description
Carbenicillin is a semi-synthetic, broad-spectrum carboxypenicillin antibiotic with bactericidal and beta-lactamase resistant activity. It is a broad-spectrum antibiotic, meaning that it is effective against a wide range of bacteria, including both Gram-positive and Gram-negative bacteria. Carbenicillin is particularly useful against Pseudomonas aeruginosa, a Gram-negative bacterium that is often resistant to other antibiotics.

Carbenicillin is commonly used in cell biology applications to prevent the growth of bacterial contaminants. It is also used in microbiology to select for bacteria that have been transformed with a vector harboring the gene encoding beta-lactamase, which makes them resistant to carbenicillin.
Application
Carbenicillin disodium salt has been used:

in the preparation of Luria-Bertani (LB) agar plates and media
as a selective agent in the culture media to prevent the growth of bacterial contaminants
in a study focused on the development of monoclonal antibodies

Biochem/physiol Actions
Mode of Action: Carboxypenicillin antibiotic that inhibits bacterial cell-wall synthesis (peptidoglycan cross-linking) by inactivating transpeptidases on the inner surface of the bacterial cell membrane.

Antimicrobial spectrum: Active against Gram-positive and Gram-negative bacteria
Carbenicillin acylates the penicillin-sensitive transpeptidase C-terminal domain by opening the lactam ring. This inactivation prevents the cross-linkage of peptidoglycan strands, thereby inhibiting the third and last stage of bacterial cell wall synthesis. This leads to incomplete bacterial cell wall synthesis and eventually causes cell lysis.
Features and Benefits
Broad-spectrum antibiotic with bactericidal and beta-lactamase resistant activity
Effective against a wide range of bacteria, including Pseudomonas aeruginosa
Commonly used in Cell Biology and Biochemical applications
Offers greater stablility than ampicillin

Preparation Note
Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
Analysis Note
Stable at 37 °C for 3 days
Other Notes
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Keep container tightly closed in a dry and well-ventilated place.