Merck, C2978-250ML, CelLytic™M

General description
CelLytic M is a proprietary detergent solution designed for efficient whole-cell protein extraction from cultured mammalian cells. It enables efficient and rapid cell lysis and solubilization of proteins for both suspension and adherent cells. Lysates can be used in many downstream applications without removing the CelLytic M such as reporter gene assays, Western blots/immunoprecipitation, electrophoretic mobility shift assays, phosphatase assays and kinase asssays.
Features and Benefits
Efficient: Up to 50% more efficient than freeze thaw, sonication and other products
Non-denaturing: Does not interfere in downstream applications such immunoprecipitation, kinase and phosphatase assays, reporter gene assays and gel shift assays
Convenient: Ready-to-use reagent requires no scraping from culture plates
Fast: Rapid cell lysis at room temperature.

Preparation Note
CelLytic M reagent efficiency for protein extraction has been tested on, but not limited to, HeLa, CHO, COS, HL-60, Jurkat, A431, PC-12, and Bovine Aorta Endothelial Cells (BAEC).
Analysis Note
Use 125 μl CelLytic M for 10⁶-10⁷ of suspended cells. For adherent cells, use 500-1,000 μL for a 100 mm plate; 200-400 μL for a 35 mm plate.
Legal Information
CelLytic is a trademark of Sigma-Aldrich Co. LLC