Merck, C3028-.5MG, Colipase from porcine pancreas

Name: Colipase from porcine pancreas
Brand: Merck
SKU: C3028-.5MG

Size:0.5 mg, Colipase from hog pancreas, essentially salt-free, lyophilized powder

Catalog Number: C3028-.5MG

Pricing

Send an RFQ.

View RFQ List

Category

Protein-Free Media

Pack Size0.5 mg

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Research Area: Cell Signaling
Colipase is a member of a group of small cysteine-rich proteins characterized by a limited secondary structure. Its structure is relatively flat, measuring 25×30×35 Å, resembling an arrangement of protruding fingers interconnected at one end by a complex network of five disulfide bridges. The colipase structure comprises two small domains with closely related topology.
The gene colipase (CLPS) encodes a lipolytic enzyme that is majorly expressed in the exocrine pancreas. This protein is also found in the stomach and intestine. Its structure includes 17 amino acid signal peptides and a five amino acid pro-piece at the N-terminus.
Application
Colipase from porcine pancreas has been used:

in the enzyme solution for lipase assay
in an enzyme mixture to simulate gastrointestinal digestion
as one of the apparent critical factors to study its effect on lycopene in vitro accessibility

Biochem/physiol Actions
Colipase is involved in lipid metabolism and apoptosis signaling. It is crucial for fat digestion. It is a pancreatic protein that prevents the denaturation of lipase and enables its attachment to the lipid-water interphase of the droplet. Colipase makes lipase accessible to the inner core of triacylglycerol. Defective colipase secretion induced by an insufficient exocrine pancreatic function minimizes the luminal hydrolysis of dietary fat. Lack of congenital lipase or colipase causes pancreatic fat malabsorption.
Colipase, a small protein cofactor, is essential for efficient dietary lipid hydrolysis by pancreatic lipase. It attaches to the non-catalytic C-terminal domain of the lipase, stabilizing an active conformation and significantly enhancing the overall hydrophobic binding site. This pancreatic exocrine protein aids in the adsorption of pancreatic triglyceride lipase (PTL) to the substrate lipid-water interface.
Features and Benefits

Overcomes the inhibition of lipase by bile salts.
Activity can be demonstrated turbidimetrically in an emulsion of triolein and sodium deoxycholate together with porcine pancreatic lipase.

Preparation Note
Dissolves in water to form a clear, colorless to faint yellow colored solution at 1.9-2.1 mg/mL concentration.

Specifications

General

UNSPSC Code	12352204
EC Number	259-490-1
NACRES	NA.54
MDL number	MFCD00130823
biological source	Porcine pancreas
assay	≥95% (Lowry)
form	essentially salt-free, lyophilized powder
concentration	≥500 μ protein/vial
color	white to faint yellow
solubility	H2O: soluble 1.9-2.1 mg/mL, clear, colorless to faintly yellow
suitability	suitable for molecular biology
UniProt accession no.	P02703
application(s)	food and beverages
storage temp.	−20°C
Quality Level	200
Gene Information	pig ... CLPS(397407)

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.

Merck, C3028-.5MG, Colipase from porcine pancreas

Send an RFQ.

General

Notice

More from Merck.

Guanidinium chloride

Tris(hydroxymethyl)aminomethane hydrochloride

D-(+)-Glucose

Tri-n-butyl phosphate

More from Merck.

Guanidinium chloride

Tris(hydroxymethyl)aminomethane hydrochloride

D-(+)-Glucose

Tri-n-butyl phosphate