Merck, C3131-1G, Cytochrome c from bovine heart

Name: Cytochrome c from bovine heart
Brand: Merck
SKU: C3131-1G

Size:1 g, CYC, CytC, ≥95% based on Mol. Wt. 12, 327 basis, powder, suitable for mammalian cell culture

Available Catalog Numbers

C3131-100MG C3131-10MG C3131-1G C3131-250MG C3131-50MG

Catalog Number: C3131-1G

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Category

Protein-Free Media

Pack Size1 g

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Research area: Apoptosis

Cytochrome c (Cyt C) is an essential mitochondrial protein located in the inner membrane of the mitochondria. It is encoded in the nucleus as apo-cytochrome C. Cyt C occurs as membrane-bound or as soluble metalloproteins in energy-transducing membranes and is found in bacteria, archaea, and plastids. It belongs to the group of class IIa cytochromes and contains pentacoordinate heme centres.
Application
Cytochrome C from bovine heart has been used:

as a component of the assay solution for testing the activity of respiratory chain complexes III and IV in the mitochondria of human osteosarcoma and human hepatocarcinoma cell lines
to determine the cytochrome c oxidase activity in mitochondria of human aortic endothelial cells
as a component of the cyt C oxidase reaction solution during in situ enzyme staining of cyt C oxidase in mice kidney tissues

Biochem/physiol Actions
Cytochrome C (Cyt C) is involved in the activation of caspase during the caspase-dependent apoptosis intrinsic pathway that triggers programmed cell death through apoptosis. It shows lipid-binding activity through its interaction with cardiolipin that accounts for the peroxidase activity of Cyt C. Cyt C binds to heme via the help of cytochrome c heme lyase that enables its release into the mitochondrial intermembrane space.
The ready fluctuation of cytochrome c within the cell between ferrous and ferric states, makes it an efficient biological electron-transporter. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal catalyst of respiration, it forms the essential electron-bridge between the respirable substrates and oxygen.
The ready interconversion of cytochrome c between ferrous and ferric states makes it an efficient biological electron carrier. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal link in the respiratory chain, it forms the essential electron-bridge between the respirable substrates and oxygen.
Preparation Note
Prepared with acetic acid without using TCA.
Other Notes
View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enzymeexplorer.

Specifications

General

UNSPSC Code	12352202
EC Number	232-700-9
NACRES	NA.61
MDL number	MFCD00130890
biological source	bovine heart
assay	≥95% based on Mol. Wt. 12,327 basis
form	powder
mol wt	12327 Da
storage condition	(Tightly closed Dry)
technique(s)	cell culture \| mammalian: suitable
impurities	0.40-0.50% Iron (anhydrous)
solubility	water: 10 mg/mL, dark red-brown
UniProt accession no.	P62894
storage temp.	−20°C
Gene Information	cow ... CYC1(512500)
Quality Level	200

Ordering

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Merck, C3131-1G, Cytochrome c from bovine heart

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