Merck, C3260-5KU, Citrate Synthase from porcine heart

Application
Citrate Synthase from porcine heart has been used:
to inject newt egg for determining its importance in egg activation at fertilization
in the preparation of reaction mix to determine pyruvate carboxylase enzyme activity
to examine whether it can induce a [Ca2+] increase and egg activation in unfertilized eggs

Biochem/physiol Actions
Citrate synthase catalyses the conversion of citrate to acetyl-CoA in the presence of coenzyme-A with the release of H2O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine.
Physical form
Suspension in 3.2 M (NH4)2SO4 solution, pH 7.0.
Other Notes
One unit will form 1.0 μmole of citrate from oxalacetate and acetyl CoA per min at pH 8.0 at 37 °C.