Merck, C4018-3KU, Choloylglycine Hydrolase from Clostridium perfringens (C. welchii)

Application
The enzyme from Sigma has been used in the analysis of bile samples in various studies.
Biochem/physiol Actions
The enzyme is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, liberates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg²⁺, Cu²⁺, and Cd²⁺) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.
Physical form
Partially purified lyophilized powder containing buffer salts and stabilizer
Other Notes
One unit will hydrolyze 1.0 μmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37 °C.