Merck, C9521-100MG, Z-Phe-Arg 7-amido-4-methylcoumarin hydrochloride

General description
A fluorogenic substrate for plasma kallikrein.
Z-Phe-Arg 7-amido-4-methylcoumarin (Z-FR-AMC) is a peptidomimetic substrate for papainand other enzymes such as cathepsin K. It is also a fluorogenic synthetic peptide for the enzymes cathepsins L and B.
Application
Z-Phe-Arg 7-amido-4-methylcoumarin hydrochloride has been used:
as a fluorogenic substrate in actinidin inhibition assay
as a kallikrein substrate
as a trypsin substrate for fluorometric assay
as a cathepsin-L substrate

Biochem/physiol Actions
Z-Phe-Arg 7-amido-4-methylcoumarin (Z-FR-AMC) proteolytic lysis by proteases leads to the liberation of AMC resulting in increased fluorescence in the enzymatic reaction.