Merck, C9801-5G, Casein, N,N-dimethylated from bovine milk

General description
Casein is a milk protein, that is not expensive and nontoxic. highly stable. This phosphoproteins accounts for 80% of the total protein present in bovine milk. Casein is a member of one of the larger family of secretory calcium?binding phosphoproteins.
Application
Casein, N,N-dimethylated from bovine milk can be used in assays to detect and help determine the functional role of tissue transglutaminase (tTG) in disease. In particular, Casein, N,N-dimethylated has been used in the mechanic and kinetic analysis of tTG on protein substrates, which may be linked to neurodegenerative disease.

This preparation has less than 10% reactivity with 2,4,6-trinitrobenzenesulfonic acid (TNBS) compared to non-methylated casein.

Protease substrate that has been used to assay basilase and a subtilisin analog that had been produced by site-directed mutagenesis.
Casein, N,N-dimethylated from bovine milk has been used:
in the determination of general proteolytic activity
as a substrate to study the effect of dephosphorylation on the calcium-activated neutral proteases (CANP) proteolysis of paired helical filaments (PHF) II-τ
in transglutaminase (TG) assay

Biochem/physiol Actions
Casein is highly preferred as a drug delivery vehicle. It possess antioxidant activity.
Preparation Note
Reductive methylation of C5890 by the method of Cabacungan, J.C., et al., Anal. Biochem., 124, 272 (1982).