Merck, C9903-2MG, Cholera Toxin B subunit

General description
Cholera Toxin is a heterohexameric AB5 enterotoxin. Cholera Toxin B subunit is the the B subunit pentamer of cholera toxin produced by Vibrio cholera. It is generally involved in the cell trafficking and signalling machineries.
Application
Cholera Toxin B subunit has been used:
for macrophage stimulation and i.p. injection in a study to determine the endotoxin sensitivity of Caspase-4.
in transganglionic and retrograde tract-tracing method combined with dual-immunofluorescence histochemistry of adult rat Vmes neuron cells.

Biochem/physiol Actions
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport.
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.
Features and Benefits
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Packaging
Package size based on protein content
Physical form
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide, and sodium EDTA.
Preparation Note
When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN₃ and 1 mM sodium EDTA.
Analysis Note
Activity measured by ELISA using ganglioside GM₁-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with 0.05-1 μg of Cholera toxin B subunit per mL.