Merck, CN406-05, Canine Osteoblasts: CnOb (Cryovial)

General description
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Canine osteoblasts provide an excellent model system for studies relevant to the skeletal system, including mechanisms of cancerogenesis.

Canine osteoblasts have been used to define a biomarker profile of canine osteosarcoma (Milovancev, 2013), elucidate the roles of oncogenic STAT3 (Fossey, 2009, 2011) and canonical Wnt signaling (Piskun, 2013) pathways, and to develop personalized osteosarcoma treatments by testing Src inhibitor dasatinib (Davis, 2013), procaspase-3 activating compound PAC-1 (Book, 2012), HSP90 inhibitor STA-1474 (McCleese, 2009) and STAT3 inhibitor LLL12 (Couto, 2012, 2013).

Characterization: Positive for bone mineralization.
Application
model for skeletal system research, signaling pathways, bone morphogenesis, gene expression, cell activation and proliferation, wound healing, bone formation and regeneration, treatment strategies, tissue engineering, in vitro bone models, mineralization, hormone treatments
Biochem/physiol Actions
Bone
Preparation Note
1st passage, >500,000 cells in Canine Osteoblast Basal Medium containing 10% FBS & 10% DMSO
Can be cultured at least 10 doublings

Please refer to the CnOb Culture Protocol.
Other Notes
Canine Osteoblast Basal Medium containing 10% FBS & 10% DMSO