Description
General description
The Taq DNA polymerase activity is inactivated by combining the enzyme with JumpStart™ Taq antibody, a neutralizing monoclonal antibody to Taq DNA polymerase. Antibody inactivation provides a simple, efficient procedure for hot-start polymerase chain reaction (PCR). Hot start PCR can significantly improve the results of DNA amplification by reducing the generation of nonspecific amplification products and primer-dimer artifacts. When used in PCR, JumpStart Taq DNA polymerase is inactive at low (room) temperature. When the temperature is raised above 70 °C in the first denaturation step of the cycling process, the complex dissociates and the polymerase becomes fully active.
Application
For PCR amplifications that require reduced non-specific amplification
For multiplex PCR
For reduction of primer dimers
JumpStart™ Taq DNA polymerase has been used:
In the PCR amplification of DNA isolated from herbarium specimens.
In the PCR reaction mixture for randomly amplified polymorphic DNA polymerase chain reaction (RAPD PCR).
To amplify and detect a point mutation in the EGFR exon 19 using specific cancer cell lines.
In real-time quantitative PCR
For PCR amplification of complex genomic or cDNA templates
For the PCR amplification of very low-copy number targets
For the PCR amplification of many thermal cycles (>35), and multiple primer pairs in the same reaction tube
Features and Benefits
Reduces non-specific amplification
Increases PCR specificity and yield
Reduces set-up time concerns associated with manual or wax Hot Start methods
Activation time of less than 1 minute
Packaging
JumpStart Taq DNA Polymerase is provided with a 10× reaction buffer available with and without MgCl₂. The magnesium free 10× buffer also includes a separate tube of 25 mM MgCl₂for optimization.
Supplied with 10× reaction buffer without MgCl₂. Includes a separate tube of 25 mM MgCl₂
Other Notes
One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.
Sigma′s JumpStart Taq DNA Polymerase is an antibody-inactivated hot-start enzyme designed to minimize non-specific amplification while increasing target yield. Once the reaction temperature reaches 70°C, Taq DNA polymerase activity is restored and the resulting PCR exhibits a higher specificity and yield. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. The enzyme may also be included in the master mix preparation resulting in more consistency from one reaction to the next.
View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.
Legal Information
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
