Merck, D4522-5MG, Deoxyribonucleic acid from calf thymus

General description
High quality double-stranded template DNA isolated from the thymus of male and female calves.
Application
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard to determine DNA content in gels and for DNA quantification by fluorescent assay.
Features and Benefits
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA
Preparation Note
Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.
One mg DNA is approx. 20 A₂₆₀ units.
Prepared from highly polymerized calf thymus DNA and nicked with DNase using the method of Aposhian and Kornberg.