General descriptionDulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains a higher concentration of amino acids, vitamins, and additional supplementary components. The original DMEM formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. This modification has been shown to improve the stability of the medium.
ApplicationDulbecco′s Modified Eagle′s Medium - low glucose has been used to culture:
- human osteosarcoma cells and HeLa cells
- primary human mesenchymal stromal cells (hBMSCs)
- human embryonic kidney cell lines
Preparation NoteSupplement with 0.584 g/L glutamine.