Merck, D6815-1SET, 3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride

Application
The DAB (3,3′-Diaminobenzidine tetrahydrochloride) Two-component Enhanced Liquid Substrate System is a precipitating substrate system for peroxidase. DAB Two-component Liquid Substrate System produces a brown precipitate for use in immunohistological and immunoblotting staining procedures.
3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride has been used as a substrate for peroxidase coupled secondary antibody.
in paraffin heart sections of mice for immunohistochemical and immunofluorescent staining
in immunotransfer of salivary protein of laboratory Lutzomyia ovallesi
in immunoperoxidase staining of lung tissue

Biochem/physiol Actions
3,3′-Diaminobenzidine (DAB) is a chromogenic substrate for peroxidase enzyme. It is converted to an insoluble precipitate after oxidation by peroxidase. This feature is exploited for visualization in histochemical (HC) and immunohistochemical (IHC) staining.
Packaging
Set consists of 4 x 100 ml Solution A and 15 ml Solution B.
Preparation Note
Each DAB Two-component Liquid Substrate System will provide 500 mL of the DAB substrate solution.
Disclaimer
Stable for up to 18 mo. when stored at 2-8 °C in the original container.