Description
General description
Dulbecco′s Modified Eagle′s medium (DMEM), a regularly used cell culture medium, is a source of energy-rich media that includes glucose, vitamins, and amino acids. A 1:1 mixture of Dulbecco′s Modified Eagle′s medium (DME) and Ham′s F-12 nutrient mixture is commonly used in cell culture research. To compensate for the loss of buffering capacity caused by removing serum, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer may be added in the formulations at a final concentration of 15 mM.
Application
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham:
has been used as a component in the culture medium to culture primary culture-activated human hepatic stellate cells
has been used to culture the cartilage biopsies
may be used for the propagation of Cowdria ruminantium
Other Notes
2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research