Description
General description
Glycerol-Free JumpStart™ Taq DNA Polymerase is a lyophilization-compatible blend of Sigma′s high-performance Taq DNA Polymerase and JumpStart™ Taq antibody. The Taq DNA polymerase is an antibody-inactivated hot-start enzyme designed to minimize non-specific amplification while increasing target yield. Once the reaction temperature reaches 70 °C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set up with less contamination risk than manual hot-start techniques. The enzyme may also be included in the master mix preparation resulting in more consistency from one reaction to the next.
Glycerol-free JumpStart™ Taq DNA Polymerase is suitable for lyophilization and allows the user to lyophilize PCR master mixes, which can be stored at ambient temperature and used up to one year later without reagent degradation.
Application
Typical applications for Glycerol-Free JumpStart™ Taq DNA polymerase include lyophilization of PCR master mixes and any other DNA amplification protocol in which glycerol is contraindicated, such as freeze-drying and automated PCR applications.
Features and Benefits
Designed to minimize non-specific amplification
Increased specificity and target yield
Reduced primer-dimers
Reduced set-up time as compared to manual or wax Hot Start methods
Allows room-temperature reaction set-up
Suitable for multiplex PCR
Lyophilization-compatible hot-start enzyme that is glycerol-free
Packaging
The enzyme is supplied at 5 units/μL in 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.05 mM EDTA, 5 mM DTT, and stabilizers.
Other Notes
One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.
View more detailed information on JumpStart Taq enzymes at our Hot Start PCR page.
Legal Information
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
