Merck, DNTP100-1KT, Deoxynucleotide Set, 100 mM

Name: Deoxynucleotide Set, 100 mM
Brand: Merck
SKU: DNTP100-1KT

Size:1 kit, DNTP Set for PCR, Individual dNTPs for routine PCR; 0.25 mL each

Catalog Number: DNTP100-1KT

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Category

dNTPs & NTPs

TagsMolecular Biology & Functional Genomics PCR & Amplification Kapa Biosystems PCR Reagents

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Deoxynucleotide triphosphates (dNTPs) are organic molecules, which contains nucleobase bound to deoxyribose. This dNTPs set includes 0.25 mL each of 100mM aqueous solutions of four deoxyribonucleotides, deoxyribose adenosine triphosphate (dATP), deoxyribose cytidine triphosphate (dCTP), deoxyribose guanosine triphosphate (dGTP), and deoxyribose thymidine triphosphate (dTTP) in separate tubes for convenience and easy PCR reaction setup.
Application
Deoxynucleotide Set, 100 mM has been used as a component of the reaction mixture for polymerase chain reaction (PCR).It has also been used as a component of the reaction mixture for amplification refractory mutation system polymerase chain reaction (ARMS-PCR) to genotype single-nucleotide polymorphisms (SNPs).
Deoxynucleotide Set, 100 mM has been used:

to biotinylate λ-DNA for magnetic tweezers measurements
in RAPD PCR
in FISH probe generation and labeling
in PCR amplification of genomic DNA
in multiplex PCR
in first-strand cDNA synthesis
in TUNEL labeling
in RT-PCR
as a component of the reaction mixture for polymerase chain reaction (PCR)

Biochem/physiol Actions
Deoxynucleotide triphosphates (dNTPs) are substrates for DNA polymerizing enzymes. They act as building blocks of nucleic acid molecules and are acidic in nature. dNTPs are key components of PCR master mixes and are highly essential to amplify DNA. Optimal levels of dNTPs ensure proper chromosomal replication and in turn maintain genome stability.
Features and Benefits

Provided as 4 separate tubes of dNTPs.
Allows optimization of individual dNTP concentrations.

Packaging
0.25 mL each of 100 mM dATP, dCTP, dGTP and dTTP

Specifications

General

NACRES	NA.52
UNSPSC Code	41106305
form	liquid
feature	PCR Additives
concentration	100 mM
technique(s)	PCR: suitable
color	colorless
shipped in	dry ice
storage temp.	−20°C
Quality Level	200

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Merck, DNTP100-1KT, Deoxynucleotide Set, 100 mM

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