Merck, DR1052-2MG, Anti-SALL4 Mouse mAb (6E3)

General description
Purified mouse monoclonal antibody. Recognizes the ~100 kDa SALL4 protein.
Recognizes the ~100 kDa SALL4 protein in HeLa and NIH3T3 cells and human testes tissue.
This Anti-SALL4 Mouse mAb (6E3) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of SALL4.
Immunogen
A recombinant polypeptide corresponding to amino acids 954-1054 of human SALL4, expressed as a GST fusion protein
NA
Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (0.2 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HeLa cells, NIH3T3 cells, Human testes tissue
Other Notes
Cauffman, G., et al. 2009. Hum. Reprod.24 63.
Forte, A., et al. 2009. Mol. Med.15 392.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 µg/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)