Description
General description
Ethylenediaminetetraacetic acid (EDTA) is a polyprotic acid. The four carboxylic acid and two amine groups with lone-pair electrons of EDTA can chelate calcium and several other metal ions.
Ethylenediaminetetraacetic acid disodium salt solution is a chelating agent suitable for molecular biology applications.
Application
Ethylenediaminetetraacetic acid disodium salt solution has been used:
in Tris hydrochloric acid (HCl) for digesting proteinase-K to determine the amount of DNA in the corneal sample
with papain to digest corneal samples for the quantification of glycosaminoglycan (GAG) content
in pretreatment for detaching epithelial cells grown to 80% confluency
to prepare stock solutions for lysis buffer
in the preparation of urea lysis buffer
Features and Benefits
molecular biology grade
Preparation Note
Prepared in 18 megohm water with Molecular Biology Reagent EDTA (E 5134)
Other Notes
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