Description
General description
The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.
Method of purification - Affinity tag purification
Immunogen
FLAG; peptide sequence DYKDDDDK
Application
For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) , immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.
Western Blotting and EIA
Learn more product details in our FLAG® application portal.
Biochem/physiol Actions
Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C
Physical form
Contains 50% glycerol in phosphate buffered saline.
Preparation Note
Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk
Other Notes
2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany