Merck, F3665-1MG, Lipopolysaccharides from Escherichia coli O111:B4

Name: Lipopolysaccharides from Escherichia coli O111:B4
Brand: Merck
SKU: F3665-1MG

Size:1 mg, LPS, FITC conjugate

Available Catalog Numbers

F3665-10MG F3665-1MG

Catalog Number: F3665-1MG

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Category

Polysaccharides

Pack Size1 mg

TagsCell Culture & Analysis Cell Culture Supplements & Reagents Carbohydrates

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

Application
FITC-Lipopolysaccharides (FITC-LPS) may be used to study processes such as the movement/transport of LPS across tissue barriers such as the colonic epithelium and cellular binding and internalization of lipopolysaccharides with fluorescence-based assay systems. It may be used to evaluate, qualify and quantify lipopolysaccharides (LPS) removal reagents and procedures. FITC-LPS conjugate may be used for the detection of LPA binding proteins and motifs.
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
Biochem/physiol Actions
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
Preparation Note
The product is soluble in water (5 mg/ml) yielding a hazy, yellow to orange solution. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution. For cell culture use, LPS should be reconstituted by adding 1 ml of sterile balanced salt solution or cell culture medium to a vial (1 mg) and swirling gently until the powder dissolves. Solutions can be further diluted to the desired working concentration with additional sterile balanced salt solutions or cell culture media.
Other Notes
To gain a comprehensive understanding of our extensive range of lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Specifications

General

UNSPSC Code	12352201
EC Number	297-473-0
NACRES	NA.25
MDL number	MFCD00164401
biological source	Escherichia coli (O111:B4)
conjugate	FITC conjugate
form	lyophilized powder
extent of labeling	2-10 μg FITC per mg LPS
color	yellow to orange
solubility	H2O: soluble 4.90-5.10 mg/mL, faintly hazy to hazy, pale yellow to yellow
storage temp.	2-8°C
Quality Level	200

Ordering

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Merck, F3665-1MG, Lipopolysaccharides from Escherichia coli O111:B4

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