Merck, F9252-500ML, Folin & Ciocalteu′s phenol reagent

Name: Folin & Ciocalteu′s phenol reagent
Brand: Merck
SKU: F9252-500ML

Size:500 mL, suitable for determination of total protein by Lowry method, 1.9-2.1 N

Available Catalog Numbers

F9252-100ML F9252-1L F9252-500ML

Catalog Number: F9252-500ML

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Category

Microbiological Stains

Pack Size500 mL

TagsClinical & Diagnostics Tissue Diagnostics Hematology Stains

CollectionsMerck Tissue Diagnostics – Universal Reagents for Histology & IHC Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Folin & Ciocalteu′s phenol reagent is most commonly used in the Lowry method for determining protein concentration. It has also been used for the quantification of total phenolics. In this method, protein is pretreated with copper(II) in a modified biuret reagent (alkaline copper solution
stabilized with sodium potassium tartrate). Addition of the phenol reagent generates chromogens that give increasing absorbance between 550-750nm. Normally, absorbance at the peak (750nm) or shoulder (660nm) are used to quantitate protein concentrations between 1-100 mg/ml while absorbance at 550nm is used to quantitate higher protein concentrations.
In the absence of copper, color intensity is determined primarily by the tyrosine and tryptophan content of the protein, and to a lesser extent by cysteine and histidine. Copper(II) has no effect on color formation by tyrosine, tryptophan, or histidine, but reduces color formation due to cysteine.
Many modifications of the original assay procedure have been published, including methods for enhancing the color development, for determining the content of insoluble proteins, and for automating the procedure. Compounds including many buffers, chelating agents, detergents, and cyclic organic compounds can interfere with the Lowry protein assay.
Folin & Ciocalteu′s phenol reagent can also be used as a spray reagent in chromatographic procedures.
Application
Folin & Ciocalteu′s phenol reagent has been used for the estimation of total phenolic content in samples.

Other Notes
Folin & Ciocalteu′s phenol reagent does not contain phenol. Rather, the reagent will react with phenols and non-phenolic reducing substances to form chromogens that can be detected spectrophotometrically.
This product is also available as part of a kit.

Specifications

General

UNSPSC Code	12171500
NACRES	NA.47
MDL number	MFCD00132625
form	liquid
concentration	1.9-2.1 N
color	clear yellow
pH	<0.5 (20 °C)
density	1.240 g/cm3 at 20 °C
suitability	suitable for determination of total protein by Lowry method
application(s)	diagnostic assay manufacturinghematologyhistology
storage temp.	room temp
Quality Level	200

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