ApplicationNew Technical Article Comparing Performance of Different EnzymesLearn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Biochem/physiol ActionsUsed for the hydrolysis of glucuronide conjugates in urinary
metabolite analysisPhysical formAqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.
Analysis NoteMany β-glucuronidases derived from mollusks also contain sulfatase activity.
Other NotesOne Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.