Merck, G112080-12X2G, DMT-dG(tac) Phosphoramidite

General description
Proligo′s DNA phosphoramidites lead to high-yield and high-quality oligonucleotides due to their high coupling efficiency. The deprotection step of automated oligonucleotide synthesis is integral to synthesis time and final product quality. The high coupling efficiency of Proligo′s DNA phosphoramidites leads to high-yield and high-quality oligonucleotides.Substitution of standard protecting groups with the labile TAC (tert.-butylphenoxyacetyl) protecting group results in ultra-fast and easydeprotection under mild conditions, suitable for oligonucleotides with baselabile monomers and reporters as well as in-situ synthesis schemes on glass surfaces.Key Features of TAC Chemistry Deprotection of the TAC group is ultra-fast: complete deprotection inconcentrated ammonia occurs within 15 minutes at 55 °C or two hours atroom temperature
Compatible with the AMA deprotection reagent (a mixture of ≥25%ammonia in water with 40% aqueous methylamine I/I, v/v)
Highly soluble in acetonitrile. No need to add co-solvents such asdimethylformamide or methylene chloride
Suitable for the synthesis of oligomers with base-labile units e.g., dyes andmodifiers, because of less exposure to ammonia and the possibility ofroom temperature deprotection
No change is required in the reagents commonly used for DNA synthesis,except that Proligo′s Fast Deprotection Cap A solution is used instead ofCap A solution
The application of dA(tac) minimizes depurination and improves thequality of oligonucleotidesDMT-dG(tac) Phosphoramidite configured for Expedite™ and PolyGen® Synthesizers.

Legal Information
Expedite is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
PolyGen is a registered trademark of PolyGen GmbH.