Description
General description
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional protein. It is basically called as a glycolytic enzyme.
Application
Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle has been used:
for measurements of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity assay
to generate a linear standard curve to analyse GAPDH activity in the experimental samples collected from mice
in anti-aggregation assays
in purified GAPDH studies
Biochem/physiol Actions
Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate into D-glycerate-1,3-bisphosphate as part of the glycolysis pathway.
Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate as part of glycolysis. It has also been shown to have roles in initiation of apoptosis, transcription activation and the shuttling of ER to Golgi vesicles>>.
Physical form
Lyophilized powder containing citrate buffer salts
Other Notes
One unit will reduce 1.0 μmole of 3-phosphoglycerate to D-glyceraldehyde 3-phosphate per min in a coupled system with 3-phosphoglyceric phosphokinase at pH 7.6 at 25 °C.