Description
General description
Research area: Cell Signaling
Galactose-1-phosphate uridyltransferase (GALT) is a galactose metabolizing enzyme that facilitates the simultaneous conversion of uridine diphosphoglucose (UDP-glucose) and galactose-1-phosphate (gal-1P) to uridine diphosphogalactose (UDP-galactose) and glucose-1-phosphate. Classic Galactosemia (CG) is an inherited metabolic condition caused by deficiency of GALT activity. GALT gene is mapped to human chromosome 9p13. Deficiency of GALT results in type 1 galactosemia.
Application
Galactose-1-phosphate Uridyltransferase from galactose-adapted yeast has been used to perform enzyme assays.
Physical form
Contains buffer salts as citrate and reduced glutathione
Other Notes
One unit will form 1.0 μmole of glucose 1-phosphate from UDP-glucose, galactose 1-phosphate and NADP⁺ per min at pH 8.7 at 25 °C as detected by a coupled system using phosphoglucomutase.